Globinexpressing E. coli cultures were grown in shake flasks at 37°C and at 200 rpm for 4 h followed by a period of 10 h at 100 rpm, on Luria-Bertani (LB) medium supplemented with 100 mg L-1 ampicillin. Cells from total of 3 L of culture were harvested, resuspended in 100 mM potassium phosphate buffer, pH 7.0, containing 1?M phenylmethanesulfonyl fluoride (PMSF) to reach an OD600 ) 600, and lysed in a French press (SLM-Aminco)
翻譯：Globinexpressing大腸桿菌文化是生長在搖瓶在37℃和200由後10小時內為100轉4小時，在盧裏亞-博爾塔尼（磅）中補充100毫克L - 1的氨芐青黴素轉速。從3文化L總收獲細胞，懸浮在100毫米的磷酸鉀緩沖液，pH值7.0含1?M phenylmethanesulfonyl氟（PMSF）達成壹項OD600）600，並在法國報刊裂解（蘇丹解放運動Aminco）
翻譯二：
All protein purification steps were performed at 4 °C. Lysates were clarified by centrifugation
and filtration (0.45?m). His-tagged Hb and flavoHb proteins were purified on an affinity column, Sephadex HisTrap chelating column (Amersham Pharmacia Biotech),and eluted stepwise in imidazole buffer (40 and 300 mM imidazole in 100 mM potassium phosphate buffer, pH 7.0) followed by a desalting column (Sephadex G25). Sample purity was estimated to be >95% for bacterial Hbs and
>90% for flavoHbs from SDS-12% polyacrylamide gels stained in Coomassie Brillant Blue
翻譯：所有蛋白質純化步驟進行，4℃裂解澄清了離心
和過濾（0.45?米）。他的標記血紅蛋白和flavoHb蛋白質純化上的親和柱，葡聚糖HisTrap螯合柱（分離液），在100毫米鉀磷酸鹽緩沖液，pH值7.0咪唑緩沖液（40和300毫米咪唑洗脫逐步）由脫鹽後列（葡聚糖達東路）。樣品純度，估計是“95％的細菌血紅蛋白
“90％的從SDS flavoHbs - 12％聚丙烯酰胺凝膠染色考馬斯亮藍
翻譯三：
FAD content was assayed by releasing FAD from flavoHb by boiling the purified protein sample for 3 min and determining the fluorescence at 520 nm with excitation at 460 nm, by comparison with pure FAD as a standard.
翻譯：基金會含量測定基金會由flavoHb，煮沸3分鐘純化的蛋白質樣品和520納米的激發熒光的決定釋放460納米，與作為標準的比較純粹的基金會。